Search results for "MICROSCOPIE CONFOCALE"
showing 5 items of 5 documents
Impact on flavour release of the structure of composite gels containing polysaccharides: Example of the fruit préparations
2006
The main objective of this study was to understand the respective rôle of the structure of a food product and of the physicochemical interactions on flavour release. For that purpose, fruit préparations hâve been chosen as model matrices. Those matrices contain a polysaccharide as gelling agent, a cross-linked waxy corn starch (1.4%), a high sucrose content (35%), an acidic buffer and a strawberry aroma (17 molécules). Four matrices were developed by modifying either the composition or the concentration in gelling agent: two matrices of similar texture containing either LM pectin or carrageenan, and two carrageenan based matrices with différent gelled structures. In a first step; two comple…
Classification par méthodes d’apprentissage supervisé et faiblement superviséd’images multimodales pour l’aide au diagnostic du lentigo malin en derm…
2021
Carried out in collaboration with the Saint-Étienne University Hospital, this work provides additional information to help the skin diagnosis by providing new decision methods on Lentigo Maligna and Lentigo Maligna Melanoma pathologies. To this end, the modalities regularly used in clinical conditions are made available to this work and are orchestrated within a multimodal process. Among image modalities, may be mentioned the clinical photography, the dermatoscopy, and the confocal reflectance microscopy. Initially, the first steps of this manuscript focus on reflectance confocal microscopy as the work in computer diagnostic assistance is relatively underdeveloped, in particular on the dete…
Exploring the diversity of listeria monocytogenes biofilm architecture by high-throughput confocal laser scanning microscopy and the predominance of …
2015
ABSTRACT Listeria monocytogenes is involved in food-borne illness with a high mortality rate. The persistence of the pathogen along the food chain can be associated with its ability to form biofilms on inert surfaces. While most of the phenotypes associated with biofilms are related to their spatial organization, most published data comparing biofilm formation by L. monocytogenes isolates are based on the quantitative crystal violet assay, which does not give access to structural information. Using a high-throughput confocal-imaging approach, the aim of this work was to decipher the structural diversity of biofilms formed by 96 L. monocytogenes strains isolated from various environments. Pr…
Influence des conditions environnementales (statique versus dynamique) sur le développement en biofilm de Listeria monocytogenes EGD-e. Developpement…
2008
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Approche tridimensionnelle de la répartition spatiale du champignon mycorhizien à arbuscule sur racines transparisées
2022
L’imagerie tridimensionnelle d’échantillons volumineux est limitée par les phénomènes de réfraction et d’absorption de la lumière par les composants tissulaires (lipides, pigments...). Afin de limiter ses phénomènes et permettre l’acquisition d’image sur toute l’épaisseur d’une racine mycorhizée, il est nécessaire de rendre transparent les échantillons. Cela est possible en dépigmentant et en homogénéisant les différents indices de réfraction au sein du tissu, via des traitements chimiques. Cette technique, appelée transparisation, permet une analyse tridimensionnelle sur échantillon entier des structures d’intérêt marquées par fluorescence. Une vingtaine de techniques différentes de clarif…